Vaccination with DNA encoding conserved influenza viral proteins
Identifieur interne : 001810 ( Main/Exploration ); précédent : 001809; suivant : 001811Vaccination with DNA encoding conserved influenza viral proteins
Auteurs : Suzanne L. Epstein [États-Unis] ; Abigail Stack [États-Unis] ; Julia A. Misplon [États-Unis] ; Chia-Yun Lo [États-Unis] ; Howard Mostowski [États-Unis] ; Jack Bennink [États-Unis] ; Kimberly A. Benton [États-Unis] ; Lynn Cooper [États-Unis] ; Athene Hodges [États-Unis] ; Kanta Subbarao [États-Unis]Source :
- International congress series [ 0531-5131 ] ; 2001.
English descriptors
- Teeft :
- Better understanding, Challenge period, Challenge virus, Effector, Elsevier science, Empty vector, Encoding, Epstein, Fresh lung lymphocytes, Immunization, Influenza, Influenza virus, International congress series, Lethal challenge, Lymphocyte, Other subset, Plasmid, Protective immunity, Protective response, Subset, Vaccination, Vaccine, Vaccines encoding, Viral, Viral genes, Virol.
Abstract
Abstract: Background: DNA vaccines encoding conserved antigens of influenza A virus can induce broad cross-protection against multiple influenza A subtypes. Better understanding of the mechanisms of this protection can help guide vaccine development. Methods: Mice were vaccinated with plasmids expressing nucleoprotein (NP) and matrix (M) given i.m., followed by lethal i.n. challenge with influenza virus. CTL were analyzed by 51Cr-release using fresh lung lymphocytes. Results: A/NP+A/M vaccination promoted viral clearance and led to recovery, at challenge virus doses that were lethal to controls. CD8+ CTL were induced and were highly specific for influenza A antigens, but were not required for protection. Depletion of both CD4+ and CD8+ T cells during the challenge period abrogated protection. A plasmid expressing influenza B/NP was used to demonstrate specificity of antibodies produced and of protection against influenza B vs. A challenge. Discussion: DNA vaccination gave protection corresponding to the influenza A vs. B inserted genes, confirming immunological specificity. For A/NP+A/M DNA, T cells are required, but either CD4+ or CD8+ T cells protected in the absence of the other subset. These findings about DNA vaccines encoding antigens conserved among viral subtypes can be applied to human vaccine development for protection against new pandemic viruses.
Url:
DOI: 10.1016/S0531-5131(01)00403-4
Affiliations:
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Better understanding of the mechanisms of this protection can help guide vaccine development. Methods: Mice were vaccinated with plasmids expressing nucleoprotein (NP) and matrix (M) given i.m., followed by lethal i.n. challenge with influenza virus. CTL were analyzed by 51Cr-release using fresh lung lymphocytes. Results: A/NP+A/M vaccination promoted viral clearance and led to recovery, at challenge virus doses that were lethal to controls. CD8+ CTL were induced and were highly specific for influenza A antigens, but were not required for protection. Depletion of both CD4+ and CD8+ T cells during the challenge period abrogated protection. A plasmid expressing influenza B/NP was used to demonstrate specificity of antibodies produced and of protection against influenza B vs. A challenge. Discussion: DNA vaccination gave protection corresponding to the influenza A vs. B inserted genes, confirming immunological specificity. For A/NP+A/M DNA, T cells are required, but either CD4+ or CD8+ T cells protected in the absence of the other subset. These findings about DNA vaccines encoding antigens conserved among viral subtypes can be applied to human vaccine development for protection against new pandemic viruses.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Epstein, Suzanne L" sort="Epstein, Suzanne L" uniqKey="Epstein S" first="Suzanne L" last="Epstein">Suzanne L. Epstein</name></region><name sortKey="Bennink, Jack" sort="Bennink, Jack" uniqKey="Bennink J" first="Jack" last="Bennink">Jack Bennink</name><name sortKey="Benton, Kimberly A" sort="Benton, Kimberly A" uniqKey="Benton K" first="Kimberly A" last="Benton">Kimberly A. Benton</name><name sortKey="Cooper, Lynn" sort="Cooper, Lynn" uniqKey="Cooper L" first="Lynn" last="Cooper">Lynn Cooper</name><name sortKey="Hodges, Athene" sort="Hodges, Athene" uniqKey="Hodges A" first="Athene" last="Hodges">Athene Hodges</name><name sortKey="Lo, Chia Yun" sort="Lo, Chia Yun" uniqKey="Lo C" first="Chia-Yun" last="Lo">Chia-Yun Lo</name><name sortKey="Misplon, Julia A" sort="Misplon, Julia A" uniqKey="Misplon J" first="Julia A" last="Misplon">Julia A. Misplon</name><name sortKey="Mostowski, Howard" sort="Mostowski, Howard" uniqKey="Mostowski H" first="Howard" last="Mostowski">Howard Mostowski</name><name sortKey="Stack, Abigail" sort="Stack, Abigail" uniqKey="Stack A" first="Abigail" last="Stack">Abigail Stack</name><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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